abstract：:The family Cichlidae is one of the most species-rich taxa in the Neotropics. However, the factors that determine these high levels of biodiversity remain unexplored. We have analyzed the morphological, cytogenetic, and molecular data from 62 specimens of a widespread cichlid, Geophagus brasiliensis, from three adjacen...

journal_title：Zebrafish

authors： Alves-Silva AP,Dergam JA

更新日期：2015-02-01 00:00:00

abstract：:The Johns Hopkins Clinical Compound Library (JHCCL), a collection of Food and Drug Administration (FDA)-approved small molecules (1400), was screened in silico for identification of novel β2AR blockers and tested for hematopoietic stem cell (HSC) expansion and radioprotection in zebrafish embryos. Docking studies, fol...

journal_title：Zebrafish

authors： Dimri M,Joshi J,Chakrabarti R,Sehgal N,Sureshbabu A,Kumar IP

更新日期：2015-02-01 00:00:00

abstract：:Individual differences (IDs) in behavior among nonhuman animals have been documented in a wide range of taxa. Although traditionally considered noise around an average, other potentially adaptive sources of phenotypic variation exist. IDs in behavior that are consistent across time and context are more recently recogn...

journal_title：Zebrafish

authors： Toms CN,Echevarria DJ

更新日期：2014-08-01 00:00:00

abstract：:Zebrafish embryos are widely used as a model to monitor the effect of chemicals on their survival and hatching at different time epochs. This experimental design generates longitudinal data in which the observations for a given subject are correlated and they are statistically independent across the subjects. This par...

journal_title：Zebrafish

authors： Aparna C,Sanjeev S,Ajit J,Shantaram K,Jayesh B,Suresh AK

更新日期：2014-08-01 00:00:00

abstract：:For many years, zebrafish have been the prototypical model for studies in developmental biology. In recent years, zebrafish has emerged as a powerful model system to study infectious diseases, including viral infections. Experiments conducted with herpes simplex virus type-1 in adult zebrafish or in embryo models are ...

journal_title：Zebrafish

authors： Antoine TE,Jones KS,Dale RM,Shukla D,Tiwari V

更新日期：2014-02-01 00:00:00

abstract：:The ability to visualise neural circuits in zebrafish in vivo is one of the most useful aspects of this model organism in neuroscience. To maintain the transparency of embryos, however, drugs, such as 1-pheyl-2-thiourea (PTU) must be added, or researchers can use mutants that do not develop pigment (e.g., the casper)....

journal_title：Zebrafish

authors： Parker MO,Brock AJ,Millington ME,Brennan CH

更新日期：2013-12-01 00:00:00

abstract：:β-Cells of the islet of Langerhans produce insulin to maintain glucose homeostasis. Self-replication of β-cells is the predominant mode of postnatal β-cell production in mammals, with about 20% of rodent β cells dividing in a 24-hour period. However, replicating β-cells are rare in adults. Induction of self-replicatio...

journal_title：Zebrafish

authors： Li M,Maddison LA,Crees Z,Chen W

更新日期：2013-06-01 00:00:00

abstract：:Zebrafish (Danio rerio) are rapidly gaining popularity in translational neuroscience and behavioral research. Physiological similarity to mammals, ease of genetic manipulations, sensitivity to pharmacological and genetic factors, robust behavior, low cost, and potential for high-throughput screening contribute to the ...

journal_title：Zebrafish

authors： Kalueff AV,Gebhardt M,Stewart AM,Cachat JM,Brimmer M,Chawla JS,Craddock C,Kyzar EJ,Roth A,Landsman S,Gaikwad S,Robinson K,Baatrup E,Tierney K,Shamchuk A,Norton W,Miller N,Nicolson T,Braubach O,Gilman CP,Pittman J

更新日期：2013-03-01 00:00:00

abstract：:The second European Zebrafish Principal Investigator (PI) Meeting was held in March, 2012, in Karlsruhe, Germany. It brought together PIs from all over Europe who work with fish models such as zebrafish and medaka to discuss their latest results, as well as to resolve strategic issues faced by this research community....

journal_title：Zebrafish

authors： Cavodeassi F,Del Bene F,Fürthauer M,Grabher C,Herzog W,Lehtonen S,Linker C,Mercader N,Mikut R,Norton W,Strähle U,Tiso N,Foulkes NS

更新日期：2013-03-01 00:00:00

abstract：:We are entering a new era in our ability to modify and edit the genomes of model organisms. Zinc finger nucleases (ZFNs) opened the door to the first custom nuclease-targeted genome engineering in the late 1990s. However, ZFNs remained out of reach for most research labs because of the difficulty of production, high c...

journal_title：Zebrafish

authors： Blackburn PR,Campbell JM,Clark KJ,Ekker SC

更新日期：2013-03-01 00:00:00

abstract：:Many assays are used in animal model systems to measure specific human disease-related behaviors. The use of both adult and larval zebrafish as a behavioral model is gaining popularity. As this work progresses and potentially translates into new treatments, we must do our best to improve the sensitivity of these assay...

journal_title：Zebrafish

authors： Philpott C,Donack CJ,Cousin MA,Pierret C

更新日期：2012-12-01 00:00:00

abstract：:The saltwater rotifer, Brachionus plicatilis, is widely used in the aquaculture industry as a prey item for first-feeding fishes due to its ease of culture, small size, rapid reproductive rate, and amenability to enrichment with nutrients. Despite the distinct advantages of this approach, rotifers have only been spora...

journal_title：Zebrafish

authors： Lawrence C,Sanders E,Henry E

更新日期：2012-09-01 00:00:00

abstract：:Most young researchers leaving the safe haven of postdoctoral life for the unchartered territory of a PI position are confronted with a multitude of new tasks. For those using fish as their favorite model system, this often includes the daunting task of setting up and running a fish facility. New PIs are expected to k...

journal_title：Zebrafish

authors： McNabb A,Scott K,Ochsenstein Ev,Seufert K,Carl M

更新日期：2012-09-01 00:00:00

abstract：:Despite the broad repertoire of loss of function (LOF) tools available for use in the zebrafish, there remains a need for a simple and rapid method that can inhibit expression of genes at later stages. RNAi would fulfill that role, and a previous report (Dong et al. 2009) provided encouraging data. The goal of this st...

journal_title：Zebrafish

authors： De Rienzo G,Gutzman JH,Sive H

更新日期：2012-09-01 00:00:00

abstract：:This study reports the first production of offspring with vitrified sperm from a live-bearing fish Xiphophorus hellerii. The overall goal of this study was to develop streamlined protocols for integration into a standardized approach for vitrification of aquatic species germplasm. The objectives were to (1) estimate a...

journal_title：Zebrafish

authors： Cuevas-Uribe R,Yang H,Daly J,Savage MG,Walter RB,Tiersch TR

更新日期：2011-12-01 00:00:00

abstract：:A study was conducted to preliminarily assess the contribution of the intestinal microflora to biotin supply in zebrafish. Biotin and avidin were added to three isonitrogenous and isocaloric purified diets to provide molar avidin: biotin ratios of 0:0 (basal diet), 0:1 (biotin-supplemented diet), and 120:0. Another di...

journal_title：Zebrafish

authors： Yossa R,Sarker PK,Vandenberg GW

更新日期：2011-12-01 00:00:00

abstract：:Our first Zebrafish Enhancer TRAP lines database (ZETRAP) generated a few years ago was a web-based system informing the scientific community about the developmental, genetic, and genomic aspects of transgenic zebrafish lines expressing the cytosolic version of EGFP. These transgenic lines were obtained in a primary s...

journal_title：Zebrafish

authors： Kondrychyn I,Teh C,Garcia-Lecea M,Guan Y,Kang A,Korzh V

更新日期：2011-12-01 00:00:00

abstract：:With worldwide rates of obesity and type-II diabetes increasing, it is essential to identify and understand the mechanisms involved during nutrient absorption and fuel allocation. Recent studies demonstrate that nutrients (e.g., lipids and carbohydrates) play a major regulatory role in gene transcription of glycolytic...

journal_title：Zebrafish

authors： Craig PM,Moon TW

更新日期：2011-09-01 00:00:00

abstract：:Although zebrafish is used as a major model species for understanding a number of biological functions and mechanisms involved, there is practically no information on the nutritional requirements of this cyprinid. The rearing practices, especially with regard to feeds and feeding, also vary considerably. To obtain bas...

journal_title：Zebrafish

authors： Kaushik S,Georga I,Koumoundouros G

更新日期：2011-06-01 00:00:00

abstract：:Aggression is a key component of the behavioral repertoire of animals that impacts on their Darwinian fitness. The available genetic tools in zebrafish make this species a promising vertebrate neurogenetic model for the study of neural circuits underlying aggressive behavior. For this purpose, a detailed characterizat...

journal_title：Zebrafish

authors： Oliveira RF,Silva JF,Simões JM

更新日期：2011-06-01 00:00:00

abstract：:This project was developed to promote understanding of how mathematics and statistical analysis are used as tools in genetic research. It gives students the opportunity to carry out hypothesis-driven experiments in the classroom: students generate hypotheses about Mendelian and non-Mendelian inheritance patterns, gath...

journal_title：Zebrafish

authors： Lindemann S,Senkler J,Auchter E,Liang JO

更新日期：2011-06-01 00:00:00

abstract：:The zebrafish has considerable potential for use as a model in the study of behavior in social systems, particularly dominance hierarchies, which are widespread in nature and can affect the lifelong success of individuals. There is, however, a paucity of information relating to the characterization of social groups an...

journal_title：Zebrafish

authors： Paull GC,Filby AL,Giddins HG,Coe TS,Hamilton PB,Tyler CR

更新日期：2010-03-01 00:00:00

abstract：:Extracellular matrix (ECM) remodeling is a process that is crucial to the development of embryos, the growth and metastasis of tumors, and wound healing and homeostasis of tissues in adults. As such, it involves dozens of gene products that are regulated by mechanisms operating at transcriptional and multiple posttran...

journal_title：Zebrafish

authors： Wyatt RA,Keow JY,Harris ND,Haché CA,Li DH,Crawford BD

更新日期：2009-12-01 00:00:00

abstract：:The zebrafish system has been established as a useful model for the study of carcinogenesis. The cytogenetic characterization of the genome is vital for furthering our understanding of the progression of the disease. Establishing a basic description of the zebrafish chromosomal karyotype and markers for each specific ...

journal_title：Zebrafish

authors： Peterson SM,Freeman JL

更新日期：2009-12-01 00:00:00

abstract：:The two LIM kinases (LIMKs) LIMK1 and LIMK2 are members of the PDZ/LIM family. These serine/threonine protein kinases are involved in actin cytoskeleton reorganization through phosphorylation and inactivation of ADF/cofilin. Different subcellular localizations of LIMK1 and LIMK2 suggest different functions. LIMK1 is i...

journal_title：Zebrafish

authors： Vlecken DH,Bagowski CP

更新日期：2009-12-01 00:00:00

abstract：:During the last few years zebrafish and other aquarium fish have become more important model system species. In zebrafish valuable biotechnological lines are constantly emerging, and the creation of cryobanks of viable fish sperm, oocytes, and embryos from those lines is a constant and important demand. Cryopreservati...

journal_title：Zebrafish

authors： Robles V,Cabrita E,Herráez MP

更新日期：2009-09-01 00:00:00

abstract：:In an effort to engage students in original research while teaching them basic molecular biology skills, we have designed a course for upper level undergraduate students and beginning graduate students that employs in situ hybridization in whole-mount zebrafish embryos to explore the concept of differential gene regul...

journal_title：Zebrafish

authors： Schmoldt A,Forecki J,Hammond DR,Udvadia AJ

更新日期：2009-06-01 00:00:00

abstract：:The zebrafish is a valuable model for teaching developmental, molecular, and cell biology; aquatic sciences; comparative anatomy; physiology; and genetics. Here we demonstrate that zebrafish provide an excellent model system to teach engineering principles. A seven-member undergraduate team in a biomedical engineering...

journal_title：Zebrafish

authors： Shen YC,Li D,Al-Shoaibi A,Bersano-Begey T,Chen H,Ali S,Flak B,Perrin C,Winslow M,Shah H,Ramamurthy P,Schmedlen RH,Takayama S,Barald KF

更新日期：2009-06-01 00:00:00

abstract：:To promote application of the fruits of zebrafish research, we need to cultivate understanding of the use of zebrafish not only in the research community but also among the general public. On Open Days at our institute, the National Institute for Environmental Studies (NIES), our zebrafish group exhibits our research ...

journal_title：Zebrafish

authors： Aoki Y

更新日期：2009-06-01 00:00:00

abstract：:Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to mi...

journal_title：Zebrafish

authors： Bill BR,Petzold AM,Clark KJ,Schimmenti LA,Ekker SC

更新日期：2009-03-01 00:00:00

abstract：:Traditional working heart preparations, based on the original Langendorff setup, are widely used experimental models that have tremendously advanced the cardiovascular field. However, these systems can be deceivingly complex, requiring the maintenance of pH with CO(2), the delivery of oxygenated perfusate, and the nee...

journal_title：Zebrafish

authors： Hecker L,Khait L,Sessions SK,Birla RK

更新日期：2008-12-01 00:00:00

abstract：:Several factors contributed to the discovery of Wnt genes. On the one hand, the development of research tools in mammalian genetics such as mouse strains with high incidence of cancer in 1920-1930s led to discovery of the mouse mammary tumor virus in 1949. This virus in turn was used in 1982 to identify int-1 (Wnt1) i...

journal_title：Zebrafish

authors： Korzh V

更新日期：2008-09-01 00:00:00

abstract：:We have constructed a low-cost aquatic animal holding system that provides an alternative to expensive, commercially available systems. Our flow-through system is especially useful for programs that are limited in space and funding. The easy assembly and maintenance of the system are advantages for the researchers who...

journal_title：Zebrafish

authors： Hohn C,Petrie-Hanson L

更新日期：2007-07-01 00:00:00

abstract：:The zebrafish, Danio rerio, has emerged as a major model organism for biomedical research, yet little is known about its natural history. We review the literature pertaining to the geographic range, biotic and abiotic habitats, and life cycle of the zebrafish. We also report our own field study to document several asp...

journal_title：Zebrafish

authors： Engeszer RE,Patterson LB,Rao AA,Parichy DM

更新日期：2007-04-01 00:00:00

abstract：:The zebrafish mainly uses the sense of light to hunt for food and avoid predators. This reliance on vision necessitates the rapid development of the visual system. Therefore the early larva already exhibits a number of visually- mediated behaviors that can be used for a genetic analysis of vision. The present article ...

journal_title：Zebrafish

authors： Fleisch VC,Neuhauss SC

更新日期：2006-01-01 00:00:00

abstract：:A review of some exciting new directions for Xiphophorus research in behavioral ecology and integrative animal behavior is presented. The primary objective of this review is to identify areas of research in this teleost genus that, if studied further, could yield insights into the manifold causes and consequences of d...

journal_title：Zebrafish

authors： Earley RL

更新日期：2006-01-01 00:00:00

abstract：:One of the strengths of the zebrafish is the ease with which in situ hybridization can be performed to determine spatial and temporal patterns of gene expression in whole embryos. Thus far, colorimetric detection methods are mainly used for these analyses. Here we describe a fluorescent in situ hybridization (FISH) pr...

journal_title：Zebrafish

authors： Clay H,Ramakrishnan L

更新日期：2005-01-01 00:00:00

abstract：:Pamela Raymond received her B.S., M.S., and Ph.D. degrees from the University of Michigan. She is currently Professor of Molecular, Cellular, and Developmental Biology in the College of Literature, Science, and the Arts at the University of Michigan. She has held academic appointments at Harvard Medical School and the...

journal_title：Zebrafish

authors： Raymond P

更新日期：2005-01-01 00:00:00

abstract：:The zebrafish had landed in Singapore as an ornamental fish long before it became a fashionable model of scientific research. By the early 1990s, however, it became a part of the local scientific landscape. During the past decade the number of groups using zebrafish as a research model increased dramatically. In June ...

journal_title：Zebrafish

authors： You MS,Korzh V

更新日期：2005-01-01 00:00:00

abstract：:Dr. Manfred Schartl is Professor in the Department of Physiological Chemistry at the Biocenter of the University of Würzburg, Germany and Adjunct Professor for Experimental Cancer Research at the University of Bergen, Norway. He pursued undergraduate studies in biology and graduate work at the University of Giebetaen,...

journal_title：Zebrafish

authors： Schartl M

更新日期：2004-01-01 00:00:00